Dr. Ruth Lehmann鈥檚 study reveals the mechanism behind mitochondrial selection, which may help researchers design future treatments for inherited diseases.
PHOTO: Juliana Thomas
Developing egg cells conduct tests to select the healthiest of their energy-making machines to be passed to the next generation. A new study in fruit flies, May 15 in Nature, shows how the testing is done.
The work focuses on mitochondria, the cellular machines that turn the sugars, fats, and proteins we eat into energy used by the body鈥檚 millions of cells. Led by researchers at NYU School of Medicine and the University of Toronto, the research team used a direct imaging technique to watch for the first time as maternal reproductive cells carefully choose which mitochondria to pass on.
鈥淥ur results confirm the theory that egg cells execute mitochondrial selection,鈥 says senior study author , a Howard Hughes Medical Institute investigator, chair of the , and director of the at 嘿嘿视频 Health. 鈥淭he findings set the stage for new approaches to the treatment of mitochondrial diseases, which include myopathies that cause muscle weakness, neurological problems, and forms of diabetes.鈥
Mitochondria are special among cellular machines in that they have their own DNA, called mitochondrial or mtDNA. Unlike the much larger DNA sets, or genomes, that reside in the nuclei of cells, mtDNA is only passed on through the mothers鈥 egg cells.
MtDNA is also more prone than nuclear DNA to develop random changes or mutations in its DNA code that build up as a person ages, but that also occur during the development of reproductive cells to cause inherited diseases in roughly 1 in 4,300 children born in the United States.
Seeing Selection
A longstanding problem faced by the field was its inability to distinguish 鈥済ood,鈥 or functional, from 鈥渂ad,鈥 or mutant, mitochondria. This hindered efforts to understand how mitochondria were sorted and inherited, says Dr. Lehmann.
For this reason, the current study was conducted in fruit flies (Drosophila melanogaster) designed to carry a mix of functional and mutant mitochondria bearing fluorescent labels that set them apart. Having many cellular features, including mitochondrial selection, in common with humans, this fly species has served over time as a key model organism in the study of biological principles.
To protect their function, mitochondria are connected into long interconnected tubes that each contain many mtDNA molecules, researchers say. Within these tubes, mitochondria that, due to genetic flaws, fail to make any of the 13 proteins important for energy production鈥攕uch as adenosine-triphosphate (ATP)鈥攃an still survive by 鈥渂orrowing鈥 functional proteins made from other healthy DNA copies in the same tube.
By visualizing the process by which fly egg cells select mitochondria, the research team revealed that this process is triggered by a carefully timed drop in levels of mitofusin, a protein that enables mitochondria to fuse. In the face of dropping mitofusin levels, mitochondria were seen to separate into fragments. On average, each of these fragments contained many fewer complete sets of mtDNA. This forced each mitochondrion to stand on its own in terms of energy production, with the fragmented mitochondria containing mutant mtDNA not producing ATP as well.
Based on this competition, bad mitochondria are eliminated, say the authors, and the pool of mitochondria in mature egg cells becomes better able to support a healthy embryo.
By watching the fluorescent probes, the research team revealed the time frame during which mutant mitochondria were eliminated during the development of egg-making cells, or oocytes, in flies. The newfound timing in turn revealed the sorting mechanism, because selection only took place during the developmental stage at which mitochondria were fragmented. The study further determined that faulty mitochondria were removed through mitophagy, a process known to label waste products for destruction, and involving the proteins Atg1 and BNIP3.
Not only was a mitofusin drop and a fragmentation phase necessary for selection against faulty mitochondria in female reproductive cells, say the authors, but it also triggered selection when artificially induced in non-reproductive cells where it does not occur naturally. This finding set the stage for studies already underway that are exploring whether inducing mitochondrial fragmentation, by blocking fusion briefly, in bodily tissues can be used like a DNA 鈥渃leanser鈥 for diseases caused by mtDNA changes that accumulate with age.
Along with Dr. Lehmann, co-first author Toby Lieber, PhD, from the Howard Hughes Medical Institute and the Kimmel Center for Biology and Medicine of the Skirball Institute, led the study. Also leading the study was co-corresponding and co-first author Thomas Hurd, PhD, a former postdoctoral fellow at NYU School of Medicine, who now has his own lab in the Department of Molecular Genetics at the University of Toronto. He has been joined there by study authors Swathi Jeedigunta and Jonathan Palozzi. The study was funded by the Howard Hughes Medical Institute, National Institutes of Health grant R37HD41900, Canadian Institutes of Health Research grant FRN 159510, and the Canada First Research Excellence Fund.
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